Poster presentation from the Annual Metabolomics Association of North America (MANA) Conference.
To improve the efficiency of metabolomics labs and extend metabolomics into new labs, Move Analytical has been developing software-defined workflows that combine a physical kit — internal standards, external calibration and QC samples, extraction reagents, and a plate stack — with a software package designed to automate system suitability testing, experimental design, real-time data checks, automatic file processing, and result visualization. Having proven this approach with a CE-MS method (MoveKit™ CE) and a reversed-phase LC-MS method targeting bile acids (MoveKit™ BA), this work explores whether it extends to a broad polar metabolite panel using HILIC-MS.
The prototype MoveKit™ HILIC kit builds on the MoveKit CE standards, adding an early-eluting, strongly-ionizing internal standard for negative ESI, and pairs them with a targeted library of roughly 500 polar metabolites in MoveApp™. Using a 12-minute gradient on a BEH amide column with an Exploris 240, the workflow demonstrated reliable detection of internal standards and resolution of closely-eluting amino acid isomers in both positive and negative ionization modes, with initial testing underway in Move Analytical’s lab and external collaborator labs.