MoveTip #5: Use free software to get the most out of your LCMS Separation
⚡ Brief Summary When translating a LC-MS method you have seen published or developing a new one, knowing the optimal flow rate and the appropriate pressure is typically an unanswered question. Never fear, a good estimate can be calculated! In this MoveTip, we share an easy method using a tool called “Capillary Flow Calculator” within the PNNL Molecular Weight Calculator, to estimate column pressure, optimal flow rate, and post-column band broadening.
🧪 What You’ll Learn to try It Yourself When using or developing a new LCMS method, you’ll learn how to: • Where to download the PNNL Molecular Weight Calculator application • Make a very good estimate of the pressure the column should be operating • Estimate the flow rate where the column may perform best for your separation
🔗 Learn More Move Analytical Website: https://www.moveanalytical.com/
Download the PNNL Molecular Weight Calculator tool: https://pnnl-comp-mass-spec.github.io/Molecular-Weight-Calculator-VB6/