MoveTip #8 – Four Tricks for Rapid LC-MS/MS Data Browsing and Curation in Skyline
⚡ Brief Summary Skyline enables a number of visualizations that help make curation of raw LC-MS/MS data fast and consistent. Here we show four of our favorite visualization tricks within Skyline in the context of a quantitative bile acids LC-MS/MS dataset, that help you quickly spot outliers which may need manual curation.
🧪 What You’ll Learn to try It Yourself You’ll learn How To: • Utilize the page layout to most efficiently spot outliers • Use the Synchronize Integration tool • Use the Ion Ratio view to spot differences in runs based on their MS/MS spectra • Group runs of the same type together in order to feel confident about reproducibility
🔗 Learn More Move Analytical Website: https://www.moveanalytical.com/
View additional long-form Skyline tutorials here: https://skyline.ms/home/software/Skyline/wiki-page.view?name=tutorials
💬 Join the Discussion What is your own favorite metabolomics tip or mass spec laboratory hack? Share it below — we might feature it in our next MoveTip!