MoveTip #9: Is Postcolumn Broadening Ruining Your Separations?
MoveTip #9: Is Postcolumn Broadening Ruining Your Metabolite Separations?
⚡ Brief Summary One of the easiest ways to ruin a hard-earned metabolite separation is to ignore what happens between the column and the mass spectrometer. While completely avoiding post-column dispersion (broadening) is unavoidable, using the correct tubing and paying attention to removal of unwanted post-column connections is a critical step to maintaining peak shape and sensitivity, to give the best results. In this MoveTip we show two examples of configurations between the column and mass spectrometer that yielded less-than-desirable results, and how we fixed them. A bonus: how can you tell if poor peak shape is coming from post-column broadening or something else?
🧪 What You’ll Learn to try It Yourself You’ll learn: • How to assess the effect of different post-column tubing configurations on peak shape • How to View chromatograms in skyline for comparative analysis of performance • To understand the effects of both tubing length and diameter in post-column dispersion • Why you may want to avoid post-column divert valves if possible • How to tell the difference between post-column broadening and dispersion from other factors
🔗 Learn More Move Analytical Website: https://www.moveanalytical.com/